MOLECULAR IDENTIFICATION OF SOME SURROUNDING SPECIES OF FAMILY BORAGINACEAE WITHIN ELBA MOUNTAIN

Document Type : Original Article

Author

Genetics and Cytology Unit, Department of Genetic Resources, Desert Research Center, Cairo, Egypt

Abstract

Floral identification has expanded to include additional aspects including the DNA barcoding technique to aid in the identification and delimitation of wild and uncertain plant species. In the current study an area with little focus, Elba Mountain in the far southern east corner of Egypt that have been explored and surveyed for the dominant plant species. The samples were collected, and were subjected to DNA extraction, PCR and sequencing using two molecular regions (chloroplast rbcL and nuclear ITS). A total of nine species belong to three plant families are reported in this work (i.e., the Capparaceae, Lamiaceace and Moraceace). Based on both BLAST search and phylogenetic analysis, the species identification procedure and comparative analysis were explained and shown while the identification decision was taken. In conclusion, six out of the nine species were identified, five were identified based on one DNA barcode sequence, and one based on the two regions. The identified and identified cases are shown and discussed in detail. This is a part of serial studies targeting the identification and delimitation of floral species dominating Elba Mountain of Egypt.
 
 

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History

  • Receive Date: 16 June 2022
  • Revise Date: 28 June 2022
  • Accept Date: 30 June 2022

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