IN VITRO PROPAGATION OF AVOCADO (PERSEA AMERICANA MILL.)

Abo El-Fadl, Reda E.; Ahmed, Manal E.; Abd Alhady, Mohamed Reda A.

doi:10.21608/ejdr.2022.133868.1102

IN VITRO PROPAGATION OF AVOCADO (PERSEA AMERICANA MILL.)

Document Type : Original Article

Authors

¹ Department of Plant Genetic Resources, Desert Research Center, Cairo, Egypt

² Department of Genetic Resources , Desert Research Center, Cairo, Egypt

³ Tissue Culture Unit, Department of Genetic Resources, Desert Research Center, El-Matareya, Cairo, Egypt

10.21608/ejdr.2022.133868.1102

Abstract

Advances in micropropagation technique have helped to produce true-to-type clones of many important horticulture plants. A procedure for micropropagation of Avocado was developed using stem segments as explant for the in vitro establishment and 60 to 100% of explants survived. The highest average shoot length was obtained on Murashing and Skoog (MS) basal medium supplemented with 1 mg/l 6-benzyl adenine (BA) and 0.5 mg/l naphthalene acetic acid (NAA). The maximum number of proliferated shoots (6.2 shoots/ explant) was obtained on MS medium supplemented with 2 mg/l BA, 0.5 mg/l NAA and 10 mg/l arginine. Meanwhile, the highest shoot length (3.7 cm) was obtained on MS medium free from growth regulators (control). Eighty percent of the shoots rooted on half strength MS medium supplemented with 4 mg/l indole butyric acid (IBA), 0.5 mg/l NAA and 160 mg/l phloroglucinol (PG). The complete plants with the maximum average root number / shoot (5) and length (9.3 cm) was obtained on medium containing 4 mg/l IBA, 0.5 mg/l NAA and 160 mg/l PG. The highest survival percentage of 70% was obtained from the plantlets when they were transferred to greenhouse conditions. Avocado can successfully micropropagated beginning with stem segments without significant damage to mother plant.

Keywords