CLONING, MOLECULAR CHARACTERIZATION AND FUNCTIONAL ANALYSIS OF THE CIS-MUUROLADIENE SYNTHASE (SgCMS) GENE FROM LEAVES OF SALVIA GUARANITICA PLANT

Document Type : Original Article

Author

Department of Genetic Resources, Desert Research Center, El-Matareya, Cairo, Egypt

Abstract

In terms of its nutritional and medical value, Salvia guaranitica is ranked among the most important medicinal and aromatic plants because of the abundance of essential active components it contains. The most important and abundant active ingredients are terpenoid molecules, particularly monoterpenes (C10) and sesquiterpenes (C15). Terpenes play a variety of tasks and possess beneficial biological traits in plants. Considering these characteristics, the current research was done to clone S. guaranitica gene for Cis-muuroladiene synthase (SgCMS, EC: 4.2.3.67). The 600 amino acid protein coded by SgCMS has an open reading frame of 1.802 base pairs. Moreover, SgCMS protein has four terpene synthase family domains which belong to terpenes and terpenoid synthase domains. We altered and overexpressed SgCMS gene in Arabidopsis thaliana to investigate the gene's function. Compared to the wild-type plants, the transgenic A. thaliana plants showed a decrease in leaf diameter, while the flowering stems started growth earlier when compared to the GUS control. Ultimately, the results of the transgenic plants' gas chromatography-mass spectrometry examination revealed that SgCMS was in charge of producing several terpene types, particularly sesquiterpene.
 

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History

  • Receive Date: 06 May 2023
  • Accept Date: 06 June 2023

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