IN VITRO PROPAGATION OF SEARSIA TRIPARTITA (UCRIA) MOFFETT AS AN ENDANGERED MEDICINAL PLANT

Authors

1 Department of Genetic Resources, Desert Research Center, El-Matareya, Cairo, Egypt

2 Department of Genetic Resources, Desert Research Center, El-Matareya, Cairo, Egypt,

Abstract

An effective protocol for micropropagation of the endangered Searsia tripartitaplant (Anacardiaceae) was developed. Shoot tips and stem node sections from the mature plant and diverse explants from seedlings were used as explants. Murashige and Skoog (MS) medium supplemented with 0.1 mg L-1naphthalene acetic acid (NAA) and 1.5 mg L-1benzyl adenine (BA) induced the highest mean length of shoots 5.8, 6.6 and 5.3 cm for shoot tips from mature plants, shoot tips and stem node sections from seedlings, respectively. This medium also provided the highest percentage of growth induction with these three explants. The medium supplemented with 0.1 mg L-1 NAA and BA at 1.0 mg L-1induced the maximum mean length of shoots (3.4 cm) with the stem sections from mature plants. A length of the shoot of 4.7 cm was gained from the hypocotyl explant with MS medium free from plant growth regulators (PGRs). MS medium supplemented with several concentrations of either BA or kinetin (Kin) were used to determine their influence on shoot multiplication. The highest rooting percentage recorded 70% on MS medium supplemented with 3.0 mg L-1 IBA with 100 mg L-1phloroglucinol (PG) and 1 g L-1activated charcoal (AC(. An average of 50–60% of the in vitroderived transplants were survived after transferring into greenhouse conditions. Five phenolic compoundswith numerous health benefits were detected by HPLC in a comparative study between mother plant, in vitromultiplied plants and acclimatized plants to determine which of them contains high amount of phenolic compounds. 

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History

  • Receive Date: 04 July 2017
  • Revise Date: 08 December 2017
  • Accept Date: 01 December 2017

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